Students tested whether their SSB proteins wrapped DNA and if they have the two DNA binding modes similar to E.coli SSB. Experiments were done using a stop flow instrument where 1 uM SSB was rapidly mixed with 20 nM DNA containing a Cy3-Cy5 FRET pair. Wrapping = high FRET, formation of the differential binding modes is determined if there is a loss in the FRET signal.
One SSB protein from Bacillus anthracis does not the two binding modes!!
One SSB protein from Bacillus anthracis does not the two binding modes!!